Human NPTXR Recombinant Rabbit Monoclonal Antibody [PSH18-77]
cat.: HA725190
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NPTXR aa 25-500 (HA211216). |
| Positive control: | Recombinant Human NPTXR protein (HA211216). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-76] to Human NPTXR antibody (Capture) (HA725189) and Recombinant Human NPTXR protein (HA211216) as the standard. The reference range value is 78.1-40,000 pg/mL. |
| Uniprot #: | SwissProt: O95502 Human |
| Alternative names: | Neuronal pentraxin receptor NPR Nptxr NPTXR_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human NPTXR matched pair antibodies Capture: HA725189, Human NPTXR Rabbit mAb [PSH18-76] Detector: HA725190, Human NPTXR Rabbit mAb [PSH18-77] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725189) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NPTXR protein (HA211216) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725190, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native NPTXR in U-87 MG extract samples based on a 1,000 µg/ml extract load. Capture: HA725189, Human NPTXR Rabbit mAb [PSH18-76] Detector: HA725190, Human NPTXR Rabbit mAb [PSH18-77] The concentrations of NPTXR measured in duplicate and interpolated from the NPTXR standard curve and corrected for sample dilution. Undiluted samples are U-87 MG 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NPTXR concentration was determined to be 28,674 pg/ml in U-87 MG. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.