Biotin Conjugated Human Neurogranin Recombinant Rabbit Monoclonal Antibody [PSH16-57]
cat.: HA725192B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-57 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Neurogranin aa 1-78 (HA211156). |
| Positive control: | Recombinant Human Neurogranin protein (HA211156). |
| Subcellular location: | Axon, cytosol, dendritic spine head, glutamatergic synapse, mitochondrial membrane, neuronal cell body, postsynaptic membrane, trans-Golgi network transport vesicle membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-56] to Human Neurogranin antibody (Capture) (HA725191) and Recombinant Human Neurogranin protein (HA210865) as the standard. The reference range value is 7.8-4,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q92686 Human |
| Alternative names: | Protein kinase C substrate RC3 Calmodulin binding protein Hng NEUG(55-78) NEUG_HUMAN Neurogranin (protein kinase C substrate) Ng NRGN Protein kinase C substrate RC3 |
Images
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Fig1:
Sandwich ELISA analysis of Human Neurogranin matched pair antibodies Capture: HA725191, Human Neurogranin Rabbit mAb [PSH16-56] Detector: HA725192, Human Neurogranin Rabbit mAb [PSH16-57] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725191) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neurogranin protein (HA211156) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725192, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.