Biotin Conjugated Human REST Recombinant Rabbit Monoclonal Antibody [PSH21-39]
cat.: HA725194B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH21-39 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human REST aa 801-1097 (HA211258). |
| Positive control: | Recombinant Human REST protein (HA211258). |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-38] to Human REST antibody (Capture) (HA725193) and Recombinant Human REST protein (HA211258) as the standard. The reference range value is 78.1-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q13127 Human |
| Alternative names: | Neural Restrictive Silencer Factor Neural-restrictive silencer factor Neuron Restrictive Silencer Factor NRSF RE1 silencing transcription factor RE1-silencing transcription factor Repressor binding to the X2 box rest REST protein REST_HUMAN X2 box repressor XBR |
Images
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Fig1:
Sandwich ELISA analysis of Human REST matched pair antibodies Capture: HA725193, Human REST Rabbit mAb [PSH21-38] Detector: HA725194, Human REST Rabbit mAb [PSH21-39] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725193) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human REST protein (HA211258) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725194, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native REST in Raji extract samples based on a 1,000 µg/ml extract load. Capture: HA725193, Human REST Rabbit mAb [PSH21-38] Detector: HA725194, Human REST Rabbit mAb [PSH21-39] The concentrations of REST were measured in duplicates, interpolated from the REST standard curve and corrected for sample dilution. Undiluted samples are Raji extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean REST concentration was determined to be 362.650 pg/ml in Raji extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.