Human CCL2 / MCP-1 Recombinant Rabbit Monoclonal Antibody [PSH18-21]
cat.: HA725195
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL2 / MCP-1 aa 24-99 (HA210787). |
| Positive control: | Recombinant Human CCL2 / MCP-1 protein (HA210787). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-22] to Human CCL2 / MCP-1 antibody (Detector) (HA725196) and Recombinant Human CCL2 / MCP-1 protein (HA210787) as the standard. The reference range value is 7.8-4,000 pg/mL. |
| Uniprot #: | SwissProt: P13500 Human |
| Alternative names: | C-C motif chemokine 2 CCL2 CCL2_HUMAN Chemokine (C C motif) ligand 2 GDCF 2 GDCF-2 GDCF2 HC11 HSMCR30 JE MCAF MCP 1 MCP-1 MCP1 MGC9434 Monocyte chemoattractant protein 1 Monocyte chemotactic and activating factor Monocyte chemotactic protein 1 Monocyte chemotactic protein-1 Monocyte secretory protein JE SCYA2 Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) Small inducible cytokine A2 Small inducible cytokine subfamily A (Cys Cys), member 2 Small-inducible cytokine A2 SMC CF SMC-CF SMCCF |
Images
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Fig1:
Sandwich ELISA analysis of human CCL2(MCP1) matched pair antibodies Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21] Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725195) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL2 / MCP-1 protein (HA210787) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725196, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native CCL2(MCP1) in stimulated and unstimulated THP-1 extract samples based on a 1,000 µg/ml extract load. Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21] Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22] THP-1 cells were treated with 80nM PMA for 24h then treated with 100ng/ml LPS for 4h then together with 1μg/ml BFA for another 3h. The concentrations of CCL2(MCP1) were measured in duplicates, interpolated from the CCL2(MCP1) standard curve and corrected for sample dilution. Undiluted samples are stimulated and unstimulated 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL2(MCP1) concentration was determined to be 1,287 pg/ml in stimulated and 207 pg/ml in unstimulated. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.