Biotin Conjugated Human CCL11/Eotaxin Recombinant Rabbit Monoclonal Antibody [PSH16-25]
cat.: HA725198B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL11/Eotaxin aa 24-97 (HA210865). |
| Positive control: | Recombinant Human CCL11/Eotaxin protein (HA210865). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-24] to Human CCL11/Eotaxin antibody (Capture) (HA725197) and Recombinant Human CCL11/Eotaxin protein (HA210865) as the standard. The reference range value is 15.6-4,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P51671 Human |
| Alternative names: | C-C motif chemokine 11 CCL 11 CCL11 CCL11_HUMAN chemokine (C-C motif) ligand 11 Chemokine C C motif ligand 11 Chemokine CC Motif Ligand 11 Chemokine ligand 11 Eosinophil chemotactic protein Eotaxin eotaxin-1 MGC22554 SCYA 11 SCYA11 Small inducible cytokine A11 Small inducible cytokine subfamily A (Cys Cys) member 11 small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) Small inducible cytokine subfamily A member 11 Small-inducible cytokine A11 |
Images
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Fig1:
Sandwich ELISA analysis of Human CCL11/Eotaxin matched pair antibodies Capture: HA725197, Human CCL11/Eotaxin Rabbit mAb [PSH16-24] Detector: HA725198, Human CCL11/Eotaxin Rabbit mAb [PSH16-25] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725197) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL11/Eotaxin protein (HA210865) protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725198, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native CCL11 in human serum samples. Capture: HA725197, Human CCL11/Eotaxin Rabbit mAb [PSH16-24] Detector: HA725198, Human CCL11/Eotaxin Rabbit mAb [PSH16-25] The concentrations of CCL11 were measured in duplicates, interpolated from the CCL11 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 122.41 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.