Biotin Conjugated Human MCP4/CCL13 Recombinant Rabbit Monoclonal Antibody [PSH15-70]
cat.: HA725200B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MCP4/CCL13 aa 17-98 (HA211153). |
| Positive control: | Recombinant Human MCP4/CCL13 protein (HA211153). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-69] to Human MCP4/CCL13 antibody (Capture) (HA725199) and Recombinant Human MCP4/CCL13 protein (HA211153) as the standard. The reference range value is 2.0-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q99616 Human |
| Alternative names: | C-C motif chemokine 13 CCL13 CCL13_HUMAN Chemokine (C C motif) ligand 13 CK beta 10 CK-beta-10 CKb10 MCP-4 MGC17134 Monocyte chemoattractant protein 4 Monocyte chemotactic protein 4 NCC 1 NCC-1 NCC1 New CC chemokine 1 SCYA13 SCYL1 short chain Small inducible cytokine A13 precursor Small inducible cytokine subfamily A (Cys Cys) member 13 Small-inducible cytokine A13 |
Images
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Fig1:
Sandwich ELISA analysis of Human MCP4/CCL13 matched pair antibodies Capture: HA725199, Human MCP4/CCL13 Rabbit mAb [PSH15-69] Detector: HA725200, Human MCP4/CCL13 Rabbit mAb [PSH15-70] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725199) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MCP4/CCL13 protein (HA211153) starting from 1,000 pg/ml to 2.0 pg/ml and detect antibody (HA725200, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native MCP4/CCL13 in human plasma samples. Capture: HA725199, Human MCP4/CCL13 Rabbit mAb [PSH15-69] Detector: HA725200, Human MCP4/CCL13 Rabbit mAb [PSH15-70] The concentrations of MCP4/CCL13 were measured in duplicates, interpolated from the MCP4/CCL13 standard curve and corrected for sample dilution. Undiluted samples are 50% human plasma . The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP4/CCL13 concentration was determined to be 45 pg/ml in human plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.