Human S100A12 Recombinant Rabbit Monoclonal Antibody [PSH17-67]
cat.: HA725215
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human S100A12 aa 1-92 (HA211137). |
| Positive control: | Recombinant Human S100A12 protein (HA211137). |
| Subcellular location: | Secreted, Cytoplasm, cytoskeleton, Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-68] to Human S100A12 antibody (Detector) (HA725216) and Recombinant Human S100A12 protein (HA211137) as the standard. The reference range value is 156-20,000 pg/mL. |
| Uniprot #: | SwissProt: P80511 Human |
| Alternative names: | CAAF1 CAGC Calcitermin Calcium-binding protein in amniotic fluid 1 Calgranulin C Calgranulin-C Calgranulin-related protein CGRP EN RAGE EN-RAGE ENRAGE Extracellular newly identified RAGE-binding protein migration inhibitory factor-related protein 6 MRP6 Neutrophil S100 protein p6 Protein S100 A12 S100 calcium binding protein A12 S100 calcium-binding protein A12 (calgranulin C) S100 calcium-binding protein A12 S100A12 S10AC_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human S100A12 matched pair antibodies Capture: HA725215, Human S100A12 Rabbit mAb [PSH17-67] Detector: HA725216, Human S100A12 Rabbit mAb [PSH17-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725215) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human S100A12 protein (HA211137) protein starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725216, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of Human S100A12 matched pair antibodies Capture: HA725215, Human S100A12 Rabbit mAb [PSH17-67] Detector: HA725216, Human S100A12 Rabbit mAb [PSH17-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725215) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human S100A12 protein (HA211137) protein starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA725216, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of native S100A12 in Human serum. Capture: HA725215, Human S100A12 Rabbit mAb [PSH17-67] Detector: HA725216, Human S100A12 Rabbit mAb [PSH17-68] Interpolated concentration of native S100A12 was measured in duplicate at different sample concentrations and interpolated from the S100A12 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean S100A12 concentration was determined to be 269,252 pg/ml in Human serum. |
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Fig4:
Interpolated concentrations of native S100A12 in Human serum. Capture: HA725215, Human S100A12 Rabbit mAb [PSH17-67] Detector: HA725216, Human S100A12 Rabbit mAb [PSH17-68] Interpolated concentration of native S100A12 was measured in duplicate at different sample concentrations and interpolated from the S100A12 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean S100A12 concentration was determined to be 257,263 pg/ml in Human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.