Human SP-D Recombinant Rabbit Monoclonal Antibody [PSH23-95]
cat.: HA725218
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-95 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SP-D aa 21-375 (HA211160). |
| Positive control: | Recombinant Human SP-D protein (HA211160). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, surface film. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-94] to Human SP-D (Capture) (HA725217) and Recombinant Human SP-D protein (HA211160) as the standard. The reference range value is 78.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: P35247 Human |
| Alternative names: | COLEC 7 COLEC7 Collectin-7 Collectin7 Lung surfactant protein D PSP D PSP-D PSP-D Surfactant protein D PSPD Pulmonary surfactant apoprotein Pulmonary surfactant associated protein D Pulmonary surfactant associated protein PSP-D Pulmonary surfactant-associated protein D SFTP 4 SFTP4 SFTPD SFTPD_HUMAN SP D SP-D Surfactant associated protein pulmonary 4 Surfactant protein D Surfactant pulmonary associated protein D |
Images
|
Fig1:
Sandwich ELISA analysis of Human SP-D matched pair antibodies Capture: HA725217, Human SP-D Rabbit mAb [PSH23-94] Detector: HA725218, Human SP-D Rabbit mAb [PSH23-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725217) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human SP-D protein (HA211160) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725218, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native SP-D in human serum samples. Capture: HA725217, Human SP-D Rabbit mAb [PSH23-94] Detector: HA725218, Human SP-D Rabbit mAb [PSH23-95] The concentrations of SP-D were measured in duplicates, interpolated from the SP-D standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 10491.8 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.