Biotin Conjugated Mouse Apolipoprotein E Recombinant Rabbit Monoclonal Antibody [PSH16-84]
cat.: HA725222B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH16-84 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Apolipoprotein E aa 19-311 (HA211210). |
| Positive control: | Recombinant Mouse Apolipoprotein E protein (HA211210). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, Extracellular vesicle, Endosome, multivesicular body. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-83] to Mouse Apolipoprotein E antibody (Capture) (HA725221) and Recombinant Mouse Apolipoprotein E protein (HA211210) as the standard. The reference range value is 3.125-200 ng/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P08226 Mouse |
| Alternative names: | AD2 Apo-E APOE APOEA Apolipoprotein E Apolipoprotein E3 ApolipoproteinE Apoprotein LDLCQ5 LPG |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Apolipoprotein E matched pair antibodies Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83] Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725221) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein E protein (HA211210) starting from 200,000 pg/ml to 2.0 pg/ml and detect antibody (HA725222, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native APOE in mouse liver, C2C12 extract samples based on a 1mg/ml extract load. Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83] Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84] The concentrations of APOE were measured in duplicates, interpolated from the APOE standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOE concentration was determined to be 813 ng/ml in mouse liver extract, There was no detected signal in C2C12 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.