Human Acetylcholinesterase Recombinant Rabbit Monoclonal Antibody [PSH17-03]
cat.: HA725224
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Acetylcholinesterase aa 32-614 (HA211178). |
| Positive control: | Recombinant Human Acetylcholinesterase protein (HA211178). |
| Subcellular location: | Synapse, Secreted, Cell membrane; Nucleus. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-02] to Human Acetylcholinesterase antibody (Capture) (HA725223) and Recombinant Human Acetylcholinesterase protein (HA211178) as the standard. The reference range value is 97.7-25,000 pg/mL. |
| Uniprot #: | SwissProt: P22303 Human |
| Alternative names: | ACEE ACES_HUMAN Acetylcholinesterase AChE Apoptosis related acetylcholinesterase ARACHE N ACHE N-ACHE YT YT blood group |
Images
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Fig1:
Sandwich ELISA analysis of Human Acetylcholinesterase matched pair antibodies Capture: HA725223, Human Acetylcholinesterase Rabbit mAb [PSH17-02] Detector: HA725224, Human Acetylcholinesterasen Rabbit mAb [PSH17-03] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725223) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Acetylcholinesterase protein (HA211178) starting from 25,000 pg/ml to 0 pg/ml and detect antibody (HA725224, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native ACHE in SH-SY5Y extract. Capture: HA725223, Human Acetylcholinesterase Rabbit mAb [PSH17-02] Detector: HA725224, Human Acetylcholinesterasen Rabbit mAb [PSH17-03] The concentrations of ACHE were measured in duplicates, interpolated from the ACHE standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 1000 ug/ml. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean ACHE concentration was determined to be 1268 pg/ml in SH-SY5Y extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.