Biotin Conjugated Human beta Synuclein Recombinant Rabbit Monoclonal Antibody [PSH19-25]
cat.: HA725226B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH19-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human beta Synuclein aa 1-134 (HA211157). |
| Positive control: | Recombinant Human beta Synuclein protein (HA211157). |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-24] to Human beta Synuclein antibody (Capture) (HA725225) and Recombinant Human beta Synuclein protein (HA211157) as the standard. The reference range value is 156.3-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q16143 Human | Q91ZZ3 Mouse | Q63754 Rat |
| Alternative names: | Beta-synuclein SNCB |
Images
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Fig1:
Sandwich ELISA analysis of human beta Synuclein matched pair antibodies Capture: HA725225, Human beta Synuclein Rabbit mAb [PSH19-24] Detector: HA725226, Human beta Synuclein Rabbit mAb [PSH19-25] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725225) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human beta Synuclein protein (HA211157) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725226, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native SNCB in Mouse brain and Rat brain lyst samples based on a 1,000 µg/ml extract load. Capture: HA725225, Human beta Synuclein Rabbit mAb [PSH19-24] Detector: HA725226, Human beta Synuclein Rabbit mAb [PSH19-25] The concentrations of SNCB were measured in duplicates, interpolated from the SNCB standard curve and corrected for sample dilution. Undiluted samples are Mouse brain lyst and Rat brain lyst. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNCB concentration was determined to be 444,479 pg/ml in Mouse brain lyst and 486,887 pg/ml in Rat brain lyst. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.