Biotin Conjugated SNAP25 Recombinant Rabbit Monoclonal Antibody [PSH17-66]
cat.: HA725228B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH17-66 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SNAP25 aa 1-206 (HA211155). |
| Positive control: | Recombinant Human SNAP25 protein (HA211155). |
| Subcellular location: | Cytoplasm, perinuclear region, Cell membrane; Lipid-anchor, Synapse, synaptosome, Photoreceptor inner segment. |
| Recommended Dilutions:
ELISA(Cap) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-66] to SNAP25 antibody (Detector) (HA725228) and Recombinant Human SNAP25 protein (HA211155) as the standard. The reference range value is 15.6-4,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P60880 Human | P60879 Mouse |
| Alternative names: | bA416N4.2 Bdr CMS18 dJ1068F16.2 FLJ23079 HGNC:11132 MGC105414 MGC139754 Resistance to inhibitors of cholinesterase 4 homolog RIC 4 RIC-4 RIC4 SEC 9 SEC9 SNAP 25 SNAP SNAP-25 SNAP-25B SNAP25 SNP 25 SNP25 SNP25_HUMAN sp SUP Super protein Synaptosomal associated 25 kDa protein Synaptosomal associated protein Synaptosomal associated protein 25 Synaptosomal associated protein 25kDa Synaptosomal-associated 25 kDa protein Synaptosomal-associated protein 25 Synaptosomal-associated protein Synaptosomal-associated protein, 25-KD |
Images
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Fig1:
Sandwich ELISA analysis of Human SNAP25 matched pair antibodies Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65] Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725227) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human SNAP25 protein (HA211155) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725228, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native SNAP25 in SH-SY5Y and PANC-1 extract samples based on a 1,000 μg/ml extract load. Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65] Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66] The concentrations of SNAP25 were measured in duplicates, interpolated from the SNAP25 standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 13% and PANC-1 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNAL25 concentration was determined to be 2,561 pg/ml in SH-SY5Y extract and undetectable in PANC-1 extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.