Biotin Conjugated Human TREM1 Recombinant Rabbit Monoclonal Antibody [PSH17-86]
cat.: HA725234B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH17-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TREM1 aa 21-205 (HA210846). |
| Positive control: | Recombinant Human TREM1 protein (HA210846). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-85] to Human TREM1 antibody (Capture) (HA725233) and Recombinant Human TREM1 protein (HA210846) as the standard. The reference range value is 15.6-8,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9NP99 Human |
| Alternative names: | CD354 OTTMUSP00000018206 TREM 1 TREM-1 TREM1 TREM1_HUMAN Triggering receptor expressed on monocytes 1 Triggering receptor expressed on myeloid cells 1 Triggering receptor TREM 1 Triggering receptor TREM1 |
Images
|
Fig1:
Sandwich ELISA analysis of Human TREM1 matched pair antibodies Capture: HA725233, Human TREM1 Rabbit mAb [PSH17-85] Detector: HA725234, Human TREM1 Rabbit mAb [PSH17-86] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725233) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TREM1 protein (HA210846) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725234, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native TREM1 in mouse kidney samples. Capture: HA725233, Human TREM1 Rabbit mAb [PSH17-85] Detector: HA725234, Human TREM1 Rabbit mAb [PSH17-86] The concentrations of TREM1 were measured in duplicates, interpolated from the TREM1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM1 concentration was determined to be 195,813 pg/ml in mouse kidney. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.