Human FGF-21 Recombinant Rabbit Monoclonal Antibody [PSH22-34]
cat.: HA725235
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FGF-21 aa 29-209 (HA210921). |
| Positive control: | Recombinant Human FGF-21 protein (HA210921). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-33] to Human FGF-21 antibody (Detector) (HA725246) and Recombinant Human FGF-21 protein (HA210921) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: Q9NSA1 Human |
| Alternative names: | FGF 21 FGF-21 Fgf21 FGF21_HUMAN FGFL Fibroblast growth factor 21 PRO10196 UNQ3115 |
Images
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Fig1:
Sandwich ELISA analysis of Human FGF-21 matched pair antibodies Capture: HA725235, Human FGF-21 Rabbit mAb [PSH22-34] Detector: HA725236, Human FGF-21 Rabbit mAb [PSH22-33] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725235) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FGF-21 protein (HA210921) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725236, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native FGF-21 in HepG2 cell culture supernatant and human serum samples. Capture: HA725235, Human FGF-21 Rabbit mAb [PSH22-34] Detector: HA725236, Human FGF-21 Rabbit mAb [PSH22-33] The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 50% and human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 1,623 pg/ml in HepG2 cell culture supernatant and 99 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.