HRP Conjugated Human Thymidine Kinase 1 / TK1 Recombinant Rabbit Monoclonal Antibody [PSH18-63]
cat.: HA725240H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH18-63 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Thymidine Kinase 1 / TK1. |
| Positive control: | Recombinant Human Thymidine Kinase 1 / TK1 protein. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-62] to Human Thymidine Kinase 1 / TK1 antibody (Capture) (HA725239) and Recombinant Human Thymidine Kinase 1 / TK1 protein as the standard. The reference range value is 195.31-25,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P04183 Human |
| Alternative names: | cytosolic KITH_HUMAN Thymidine kinase 1 Thymidine kinase 1 soluble Thymidine kinase 1 soluble isoform Thymidine kinase Thymidine kinase cytosolic TK 1 TK1 |
Images
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Fig1:
Sandwich ELISA analysis of Human Thymidine Kinase 1 / TK1 matched pair antibodies Capture: HA725239, Human Thymidine Kinase 1 / TK1 Rabbit mAb [PSH18-62] Detector: HA725240, Human Thymidine Kinase 1 / TK1 Rabbit mAb [PSH18-63] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725239) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human TK1 protein starting from 25,000 pg/ml to 0 pg/ml and detect antibody (HA725240, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.