Human DKK1 Recombinant Rabbit Monoclonal Antibody [PSH21-08]
cat.: HA725242
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human DKK1 aa 32-266 (HA211040). |
| Positive control: | Recombinant Human DKK1 protein (HA211040). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-07] to Human DKK1 antibody (Capture) (HA725241) and Recombinant Human DKK1 protein (HA211040) as the standard. The reference range value is 11.7-6,000 pg/mL. |
| Uniprot #: | SwissProt: O94907 Human |
| Alternative names: | Dickkopf 1 Dickkopf 1 homolog Dickkopf 1 like Dickkopf homolog 1 Dickkopf like protein 1 Dickkopf related protein 1 Dickkopf WNT signaling pathway inhibitor 1 Dickkopf-1 Dickkopf-related protein 1 DKK 1 Dkk-1 Dkk1 DKK1_HUMAN hDkk 1 hDkk-1 SK |
Images
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Fig1:
Sandwich ELISA analysis of Human DKK1 matched pair antibodies Capture: HA725241, Human DKK1 Rabbit mAb [PSH21-07] Detector: HA725242, Human DKK1 Rabbit mAb [PSH21-08] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725241) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DKK1 protein (HA211040). starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA725242, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native DKK1 in HT-29 supernatant samples. Capture: HA725241, Human DKK1 Rabbit mAb [PSH21-07] Detector: HA725242, Human DKK1 Rabbit mAb [PSH21-08] The concentrations of DKK1 were measured in duplicates, interpolated from the DKK1 standard curve and corrected for sample dilution. Undiluted samples are HT-29 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK1 concentration was determined to be 30430 pg/ml in HT-29 supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.