Human CXCL2/MIP-2 Recombinant Rabbit Monoclonal Antibody [PSH17-89]
cat.: HA725248
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CXCL2/MIP-2 aa 35-107 (HA210904). |
| Positive control: | Recombinant Human CXCL2/MIP-2 protein (HA210904). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-88] to Human CXCL2/MIP-2 antibody (Capture) (HA725247) and Recombinant Human CXCL2/MIP-2 protein (HA210904) as the standard. The reference range value is 15.6-1,000 pg/mL. |
| Uniprot #: | SwissProt: P19875 Human |
| Alternative names: | C-X-C motif chemokine 2 Chemokine (C X C motif) ligand 2 Chemokine, CXC motif, ligand 2 CINC 2a CINC2a CINC3 CXC chemokine CXCL 2 Cxcl2 CXCL2_HUMAN Cytokine-induced neutrophil chemoattractant 3 GRO 2 GRO b GRO protein, beta Gro-beta GRO-beta(5-73) GRO-beta-T GRO2 GRO2 oncogene GROb GRObeta Growth regulated protein beta Growth-regulated protein beta GROX Hematopoietic synergistic factor HSF Macrophage inflammatory protein 2 alpha Macrophage inflammatory protein 2 Macrophage inflammatory protein 2-alpha Melanoma growth stimulatory activity beta MGSA b MGSA beta MIP 2 MIP 2a MIP2 alpha MIP2 MIP2-alpha MIP2A MIP2alpha SB-251353 SCYB 2 Scyb SCYB2 Small inducible cytokine subfamily B, member 2 |
Images
|
Fig1:
Sandwich ELISA analysis of Human CXCL2/MIP-2 matched pair antibodies Capture: HA725247, Human CXCL2/MIP-2 Rabbit mAb [PSH17-88] Detector: HA725248, Human CXCL2/MIP-2 Rabbit mAb [PSH17-89] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725247) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL2/MIP-2 protein (HA210904) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725248, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of spiked CXCL2 in human cell culture media samples. Capture: HA725247, Human CXCL2/MIP-2 Rabbit mAb [PSH17-88] Detector: HA725248, Human CXCL2/MIP-2 Rabbit mAb [PSH17-89] The concentrations of CXCL2 were measured in duplicates, interpolated from the CXCL2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.