Biotin Conjugated Human KIM-1/HAVCR1 Recombinant Rabbit Monoclonal Antibody [PSH18-81]
cat.: HA725250B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH18-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human KIM-1/HAVCR1 aa 21-295 (HA211313). |
| Positive control: | Recombinant Human KIM-1/HAVCR1 protein (HA211313). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-80] to Human KIM-1/HAVCR1 antibody (Capture) (HA725249) and Recombinant Human KIM-1/HAVCR1 protein (HA211313) as the standard. The reference range value is 54.7-7,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q96D42 Human |
| Alternative names: | Hepatitis A virus cellular receptor 1 HAVcr-1 Kidney injury molecule 1 (KIM-1) T-cell immunoglobulin and mucin domain-containing protein 1 (TIMD-1) T-cell immunoglobulin mucin receptor 1 (TIM; TIM-1) T-cell membrane protein 1 CD365 HAVCR1 KIM1 TIM1 TIMD1 |
Images
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Fig1:
Sandwich ELISA analysis of Human KIM-1/HAVCR1 matched pair antibodies Capture: HA725249, Human KIM-1/HAVCR1 Rabbit mAb [PSH18-80] Detector: HA725250, Human KIM-1/HAVCR1 Rabbit mAb [PSH18-81] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725249) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human KIM-1/HAVCR1 protein (HA211313) starting from 7,000 pg/ml to 0 pg/ml and detect antibody (HA725250, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native HAVCR1 in human urine samples. Capture: HA725249, Human KIM-1/HAVCR1 Rabbit mAb [PSH18-80] Detector: HA725250, Human KIM-1/HAVCR1 Rabbit mAb [PSH18-81] The concentrations of HAVCR1 were measured in duplicates, interpolated from the HAVCR1 standard curve and corrected for sample dilution. Undiluted samples are human urine 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HAVCR1 concentration was determined to be 685 pg/ml in human urine. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.