Biotin Conjugated Human IL-5RA Recombinant Rabbit Monoclonal Antibody [PSH24-51]
cat.: HA725254B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH24-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-5RA aa 21-335 (HA210727). |
| Positive control: | Recombinant Human IL-5RA protein (HA210727). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-50] to Human IL-5RA (Capture) (HA725253) and Recombinant Human IL-5RA protein (HA210727) as the standard. The reference range value is 3.9-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q01344 Human |
| Alternative names: | CD125 CD125 antigen CDw125 HSIL5R3 IL-5 receptor subunit alpha IL-5R subunit alpha IL-5R-alpha IL-5RA IL5R IL5RA IL5RA_HUMAN Interleukin 5 Receptor alpha Interleukin 5 Receptor alpha chain Interleukin 5 receptor alpha subunit Interleukin 5 receptor subunit alpha Interleukin 5 receptor type 3 Interleukin-5 receptor subunit alpha MGC26560 |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-5RA matched pair antibodies Capture: HA725253, Human IL-5RA Rabbit mAb [PSH24-50] Detector: HA725254, Human IL-5RA Rabbit mAb [PSH24-51] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725253) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-5RA protein (HA210727) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725254, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL-5RA in human plasma samples. Capture: HA725253, Human IL-5RA Rabbit mAb [PSH24-50] Detector: HA725254, Human IL-5RA Rabbit mAb [PSH24-51] The concentrations of IL-5RA were measured in duplicates, interpolated from the IL-5RA standard curve and corrected for sample dilution. Undiluted samples are human plasma 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5RA concentration was determined to be 350.1 pg/ml in human plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.