Biotin Conjugated Human IFNW1 Recombinant Rabbit Monoclonal Antibody [PSH20-77]
cat.: HA725266B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-77 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human IFNW1 aa 22-195 (HA211271). |
| Positive control: | Recombinant Human IFNW1 protein (HA211271). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-76] to Human IFNW1 antibody (Capture) (HA725265) and Recombinant Human IFNW1 protein (HA211271) as the standard. The reference range value is 15.6-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P05000 Human |
| Alternative names: | IFNW1 IFNW1_HUMAN Interferon alpha II 1 Interferon alpha-II-1 Interferon omega 1 Interferon omega-1 |
Images
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Fig1:
Sandwich ELISA analysis of Human IFNW1 matched pair antibodies Capture: HA725265, Human IFNW1 Rabbit mAb [PSH20-76] Detector: HA725266, Human IFNW1 Rabbit mAb [PSH20-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725265) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IFNW1 protein (HA211271) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725266, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.