Human IL-17B Recombinant Rabbit Monoclonal Antibody [PSH18-65]
cat.: HA725268
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-17B aa 21-180 (HA211270). |
| Positive control: | Recombinant Human IL-17B protein (HA211270). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-64] to Human IL-17B antibody (Capture) (HA725267) and Recombinant Human IL-17B protein (HA211270) as the standard. The reference range value is 5.9-1,500 pg/mL. |
| Uniprot #: | SwissProt: Q9UHF5 Human |
| Alternative names: | Cytokine like protein Zcyto7 Cytokine Zcyto7 IL 17B IL 20 IL-17B IL-20 IL17B IL17B_HUMAN IL20 Interleukin 17 beta Interleukin 17B Interleukin 20 Interleukin-17B Interleukin-20 MGC138900 MGC138901 Neuronal interleukin 17 related factor Neuronal interleukin-17-related factor NIRF ZCYTO7 |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-17B matched pair antibodies Capture: HA725267, Human IL-17B Rabbit mAb [PSH18-64] Detector: HA725268, Human IL-17B Rabbit mAb [PSH18-65] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725267) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human IL-17B (HA211270) protein starting from 1,500 pg/ml to 0 pg/ml and detect antibody (HA725268, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL-17B in HepG2 cell culture supernatant and MCF7 cell culture supernatant: Capture: HA725267, Human IL-17B Rabbit mAb [PSH18-64] Detector: HA725268, Human IL-17B Rabbit mAb [PSH18-65] The concentrations of IL-17B were measured in duplicates, interpolated from the IL-17B standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 50% and MCF7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17B concentration was determined to be 19.7 pg/ml in HepG2 cell culture supernatant and 0 pg/ml in MCF7 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.