Human IL-17C Recombinant Rabbit Monoclonal Antibody [PSH22-61]
cat.: HA725269
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-17C aa 19-197 (HA211315). |
| Positive control: | Recombinant Human IL-17C protein (HA211315). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-62] to Human IL-17C antibody (Detector) (HA725270) and Recombinant Human IL-17C protein (HA211315) as the standard. The reference range value is 31.25-8,000 pg/mL. |
| Uniprot #: | SwissProt: Q9P0M4 Human |
| Alternative names: | CX2 Cytokine CX2 IL 17C IL 21 IL-17C IL17C IL17C_HUMAN Interleukin 17C Interleukin-17C MGC126884 MGC138401 |
Images
|
Fig1:
Sandwich ELISA analysis of human IL17C matched pair antibodies Capture: HA725269, Human IL-17C Rabbit mAb [PSH22-61] Detector: HA725270, Human IL-17C Rabbit mAb [PSH22-62] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725269) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-17C protein (HA211315) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725270, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.