Biotin Conjugated Human IL-19 Recombinant Rabbit Monoclonal Antibody [PSH23-29]
cat.: HA725272B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH23-29 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-19 aa 25-177 (HA211269). |
| Positive control: | Recombinant Human IL-19 protein (HA211269). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-28] to Human IL-19 (Capture) (HA725271) and Recombinant Human IL-19 protein (HA211269) as the standard. The reference range value is 93.8-6,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9UHD0 Human |
| Alternative names: | IL 10C IL 19 IL-19 Il19 IL19_HUMAN Interleukin 19 Interleukin-19 MDA1 Melanoma differentiation-associated protein-like protein NG.1 ZMDA1 |
Images
|
Fig1:
Sandwich ELISA analysis of Human IL-19 matched pair antibodies Capture: HA725271, Human IL-19 Rabbit mAb [PSH23-28] Detector: HA725272, Human IL-19 Rabbit mAb [PSH23-29] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725271) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-19 protein (HA211269) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA725272, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native IL-19 in human milk samples. Capture: HA725271, Human IL-19 Rabbit mAb [PSH23-28] Detector: HA725272, Human IL-19 Rabbit mAb [PSH23-29] The concentrations of IL-19 were measured in duplicates, interpolated from the IL-19 standard curve and corrected for sample dilution. Undiluted samples are human milk 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-19 concentration was determined to be 380.17 ng/ml in human milk. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.