Biotin Conjugated Human/Mouse IL-20 Recombinant Rabbit Monoclonal Antibody [PSH21-86]
cat.: HA725274B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH21-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-20 aa 25-176. |
| Positive control: | Recombinant Human IL-20 protein. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-85] to Human/Mouse IL-20 antibody (Capture) (HA725273) and Recombinant Human IL-20 protein as the standard. The reference range value is 9.8-5,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9NYY1 Human |
| Alternative names: | Cytokine Zcyto10 Four alpha helix cytokine IL-20 IL10D IL20 IL20_HUMAN Interleukin 20 Interleukin-20 MGC96907 UNQ852/PRO1801 ZCYTO10 |
Images
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Fig1:
Sandwich ELISA analysis of Human IL20 matched pair antibodies Capture: HA725273, Human/Mouse IL-20 Rabbit mAb [PSH21-85] Detector: HA725274, Human/Mouse IL-20 Rabbit mAb [PSH21-86] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725273) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-20 protein starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA725274, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native IL20 in mouse serum samples. Capture: HA725273, Human/Mouse IL-20 Rabbit mAb [PSH21-85] Detector: HA725274, Human/Mouse IL-20 Rabbit mAb [PSH21-86] The concentrations of IL20 were measured in duplicates, interpolated from the IL20 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL20 concentration was determined to be 89.51pg/ml in mouse serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.