Human CCL16 Recombinant Rabbit Monoclonal Antibody [PSH19-90]
cat.: HA725281
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL16 aa 24-120 (HA211292). |
| Positive control: | Recombinant Human CCL16 protein (HA211292). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-91] to Human CCL16 antibody (Detector) (HA725282) and Recombinant Human CCL16 protein (HA211292) as the standard. The reference range value is 15.6-1,000 pg/mL. |
| Uniprot #: | SwissProt: O15467 Human |
| Alternative names: | C-C motif chemokine 16 CCL16 CCL16_HUMAN Chemokine (C-C motif) ligand 16 Chemokine CC-4 Chemokine LEC CKb12 HCC-4 HCC4 IL-10-inducible chemokine IL10 inducible chemokine IL10 inducible chemokine ILINCK LCC-1 LCC1 Liver CC chemokine 1 precursor Liver expressed chemokine Liver-expressed chemokine LMC Lymphocyte and monocyte chemoattractant Monotactin 1 Monotactin-1 MTN-1 Mtn1 NCC-4 NCC4 New CC chemokine 4 SCYA16 SCYL4 Small inducible cytokine A16 precursor Small inducible cytokine A16 precursor Small inducible cytokine subfamily A (Cys Cys) member 16 Small-inducible cytokine A16 |
Images
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Fig1:
Sandwich ELISA analysis of Human CCL16 matched pair antibodies Capture: HA725281, Human CCL16 Rabbit mAb [PSH19-90] Detector: HA725282, Human CCL16 Rabbit mAb [PSH19-91] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725281) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL16 protein (HA211292) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725282, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL16 in human plasma samples. Capture: HA725281, Human CCL16 Rabbit mAb [PSH19-90] Detector: HA725282, Human CCL16 Rabbit mAb [PSH19-91] The concentrations of CCL16 were measured in duplicates, interpolated from the CCL16 standard curve and corrected for sample dilution. Undiluted samples are human plasma 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL16 concentration was determined to be 3740.812 pg/ml in human plasma. |
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Fig3:
Interpolated concentrations of native CCL16 in Hep G2 samples. Capture: HA725281, Human CCL16 Rabbit mAb [PSH19-90] Detector: HA725282, Human CCL16 Rabbit mAb [PSH19-91] The concentrations of CCL16 were measured in duplicates, interpolated from the CCL16 standard curve and corrected for sample dilution. Undiluted samples are Hep G2 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL16 concentration was determined to be 305.002 pg/ml in HepG2 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.