Biotin Conjugated Human MCP-2 / CCL8 Recombinant Rabbit Monoclonal Antibody [PSH21-48]
cat.: HA725284B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH21-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human MCP-2 / CCL8 aa 24-99 (HA211228). |
| Positive control: | Recombinant Human MCP-2 / CCL8 protein (HA211228). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-47] to Human MCP-2 / CCL8 antibody (Capture) (HA725283) and Recombinant Human MCP-2 / CCL8 protein (HA211228) as the standard. The reference range value is 15.6-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P80075 Human |
| Alternative names: | Ccl8 CCL8_HUMAN HC14 MCP-2 MCP-2(6-76) Monocyte chemoattractant protein 2 Monocyte chemotactic protein 2 SCYA10 SCYA8 Small-inducible cytokine A8 |
Images
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Fig1:
Sandwich ELISA analysis of Human MCP-2 / CCL8 matched pair antibodies Capture: HA725283, Human MCP-2 / CCL8 Rabbit mAb [PSH21-47] Detector: HA725284, Human MCP-2 / CCL8 Rabbit mAb [PSH21-48] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725283) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MCP-2 / CCL8 protein (HA211228) starting from 1,000 pg/ml to 15.6 pg/ml and detect antibody (HA725284, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native CCL8 in PHA treated human PBMC supernatant. Capture: HA725283, Human MCP-2 / CCL8 Rabbit mAb [PSH21-47] Detector: HA725284, Human MCP-2 / CCL8 Rabbit mAb [PSH21-48] The concentrations of CCL8 were measured in duplicates, interpolated from the CCL8 standard curve and corrected for sample dilution. Undiluted samples are human PBMC 5% treated with or without 10 ug/ml PHA for 5 days. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL8 concentration was determined to be 10,262 pg/ml in PHA treated human PBMC supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.