Human GRO gamma / CXCL3 Recombinant Rabbit Monoclonal Antibody [PSH21-41]
cat.: HA725286
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human GRO gamma / CXCL3 aa 35-107 (HA211249). |
| Positive control: | Recombinant Human GRO gamma / CXCL3 protein (HA211249). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-40] to Human GRO gamma / CXCL3 antibody (Capture) (HA725285) and Recombinant Human GRO gamma / CXCL3 protein (HA211249) as the standard. The reference range value is 3.9-500 pg/mL. |
| Uniprot #: | SwissProt: P19876 Human |
| Alternative names: | C-X-C motif chemokine 3 Growth-regulated protein gamma GRO-gamma Macrophage inflammatory protein 2-beta MIP2-beta CXCL3 GRO3 GROG SCYB3 |
Images
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Fig1:
Sandwich ELISA analysis of Human GRO gamma / CXCL3 matched pair antibodies Capture: HA725285, Human GRO gamma / CXCL3 Rabbit mAb [PSH21-40] Detector: HA725286, Human GRO gamma / CXCL3 Rabbit mAb [PSH21-41] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725285) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GRO gamma / CXCL3 protein (HA211249) starting from 500 pg/ml to 0 pg/ml and detect antibody (HA725286, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CXCL3 in human serum samples. Capture: HA725285, Human GRO gamma / CXCL3 Rabbit mAb [PSH21-40] Detector: HA725286, Human GRO gamma / CXCL3 Rabbit mAb [PSH21-41] The concentrations of CXCL3 were measured in duplicates, interpolated from the CXCL3 standard curve and corrected for sample dilution. Undiluted samples are human serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 1,178 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.