Human GCSF Receptor Recombinant Rabbit Monoclonal Antibody [PSH18-04]
cat.: HA725292
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GCSF Receptor aa 25-621 (HA211262). |
| Positive control: | Recombinant Human GCSF Receptor protein (HA211262). |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-03] to Human GCSF Receptor antibody (Capture) (HA725291) and Recombinant Human GCSF Receptor protein (HA211262) as the standard. The reference range value is 31.3-4,000 pg/mL. |
| Uniprot #: | SwissProt: Q99062 Human |
| Alternative names: | CD 114 CD114 CD114 antigen Colony stimulating factor 3 receptor (granulocyte) Colony stimulating factor 3 receptor CSF 3R CSF3R CSF3R_HUMAN Csfgr G CSF R G-CSF receptor G-CSF-R GCSFR Granulocyte colony stimulating factor receptor Granulocyte colony-stimulating factor receptor OTTHUMP00000009703 OTTHUMP00000009704 OTTHUMP00000009705 |
Images
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Fig1:
Sandwich ELISA analysis of Human G-CSF-R matched pair antibodies Capture: HA725291, Human GCSF Receptor Rabbit mAb [PSH18-03] Detector: HA725292, Human GCSF Receptor Rabbit mAb [PSH18-04] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725291) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GCSF Receptor protein (HA211262) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725292, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native G-CSF-R in THP-1 and MCF7 extract samples based on a 1,000 µg/ml extract load. Capture: HA725291, Human GCSF Receptor Rabbit mAb [PSH18-03] Detector: HA725292, Human GCSF Receptor Rabbit mAb [PSH18-04] The concentrations of G-CSF-R were measured in duplicates, interpolated from the G-CSF-R standard curve and corrected for sample dilution. Undiluted samples are THP-1 extract 100% and MCF7 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean G-CSF-R concentration was determined to be 525.2 pg/ml in THP-1 extract and undetectable in MCF7 extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.