HRP Conjugated Human CXADR Recombinant Rabbit Monoclonal Antibody [PSH17-47]
cat.: HA725294H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH17-47 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CXADR aa 20-237 (HA211206) |
| Positive control: | Recombinant Human CXADR protein (HA211206). |
| Subcellular location: | Cell junction, Cell membrane, Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-46] to Human CXADR antibody (Capture) (HA725293) and Recombinant Human CXADRn protein (HA211206) as the standard. The reference range value is 625-20,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P78310 Human |
| Alternative names: | 46 kD coxsackievirus and adenovirus receptor CAR protein 46 kD coxsackievirus and adenovirus receptor protein CAR CAR4/6 Coxsackie virus and adenovirus receptor Coxsackie virus B receptor Coxsackievirus and adenovirus receptor Coxsackievirus B adenovirus receptor Coxsackievirus B-adenovirus receptor CVB3 binding protein CVB3-binding protein Cxadr CXADR Ig like cell adhesion molecule CXAR_HUMAN hCAR HCVADR |
Images
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Fig1:
Sandwich ELISA analysis of Human CXADR matched pair antibodies Capture: HA725293, Human CXADR Rabbit mAb [PSH17-46] Detector: HA725294, Human CXADR Rabbit mAb [PSH17-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725293) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXADR protein (HA211206) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725294, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.