Human IL-10RB Recombinant Rabbit Monoclonal Antibody [PSH17-44]
cat.: HA725295
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-10RB aa 20-220 (HA211205). |
| Positive control: | Recombinant Human IL-10RB protein (HA211205). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-45] to Human IL-10RB antibody (Detector) (HA725296) and Recombinant Human IL-10RB protein (HA211205) as the standard. The reference range value is 19.5-10,000 pg/mL. |
| Uniprot #: | SwissProt: Q08334 Human |
| Alternative names: | Cd210 CDw210b CRF2 4 CRF2-4 CRFB4 Cytokine receptor class II Cytokine receptor class II CRF2 4 Cytokine receptor class-II member 4 Cytokine receptor family 2 member 4 Cytokine receptor family II member 4 D21S58 D21S66 I10R2_HUMAN IL-10 receptor subunit beta IL-10R subunit 2 IL-10R subunit beta IL-10R2 IL-10RB IL10R2 IL10RB Interleukin 10 receptor beta Interleukin-10 receptor subunit 2 Interleukin-10 receptor subunit beta |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-10RB matched pair antibodies Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44] Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA725295 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-10RB (HA211205) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725296, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL-10RB in THP-1 cell extract: Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44] Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45] The concentrations of IL-10RB were measured in duplicates, interpolated from the IL-10RB standard curve and corrected for sample dilution. Undiluted samples are THP-1 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10RB concentration was determined to be 861.6 pg/ml in THP-1 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.