Human IL-12RB1 Recombinant Rabbit Monoclonal Antibody [PSH18-83]
cat.: HA725298
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-83 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-12RB1 aa 24-540 (HA210728). |
| Positive control: | Recombinant Human IL-12RB1 protein (HA210728). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-82] to Human IL-12RB1 antibody (Capture) (HA725297) and Recombinant Human IL-12RB1 protein (HA210728) as the standard. The reference range value is 78.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: P42701 Human |
| Alternative names: | CD212 cluster of differentiation 212 I12R1_HUMAN IL-12 receptor beta component IL-12 receptor subunit beta-1 IL-12R subunit beta-1 IL-12R-beta-1 IL-12RB1 IL12 receptor beta component IL12RB Il12rb1 IMD3 IMD30 Interleukin 12 receptor beta 1 chain Interleukin 12 receptor subunit beta 1 Interleukin-12 receptor subunit beta-1 MGC34454 |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-12RB1 matched pair antibodies Capture: HA725297, Human IL-12RB1 Rabbit mAb [PSH18-82] Detector: HA725298, Human IL-12RB1 Rabbit mAb [PSH18-83] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725297) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-12RB1 protein (HA210728) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725298, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL12RB1 in THP-1 extract samples based on a 1,000 µg/ml extract load. Capture: HA725297, Human IL-12RB1 Rabbit mAb [PSH18-82] Detector: HA725298, Human IL-12RB1 Rabbit mAb [PSH18-83] The concentrations of IL12RB1 were measured in duplicates, interpolated from the IL12RB1 standard curve and corrected for sample dilution. Undiluted samples are THP-1 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL12RB1 concentration was determined to be 2,380 pg/ml in THP-1 extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.