Human NKG2D Recombinant Rabbit Monoclonal Antibody [PSH17-04]
cat.: HA725299
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NKG2D aa 73-216 (HA211290). |
| Positive control: | Recombinant Human NKG2D protein (HA211290). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-05] to Human NKG2D antibody (Detector) (HA725300) and Recombinant Human NKG2D protein (HA211290) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: P26718 Human |
| Alternative names: | CD314 CD314 antigen D12S2489E Killer cell lectin like receptor subfamily K member 1 Killer cell lectin-like receptor subfamily K member 1 KLR KLRC4 KLRK1 readthrough KLRK1 NK cell receptor D NK lectin-like receptor NKG2 D activating NK receptor NKG2 D type II integral membrane protein NKG2-D NKG2-D type II integral membrane protein NKG2-D-activating NK receptor Nkg2d NKG2D_HUMAN NKLLR NKR P2 Nkrp2 |
Images
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Fig1:
Sandwich ELISA analysis of human NKGD2(KLRK1) matched pair antibodies Capture: HA725299, Human NKG2D Rabbit mAb [PSH17-04] Detector: HA725300, Human NKG2D Rabbit mAb [PSH17-05] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725299) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NKG2D protein (HA211290) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725300, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.