Human TACI / TNFRSF13B Recombinant Rabbit Monoclonal Antibody [PSH18-67]
cat.: HA725302
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TACI / TNFRSF13B aa 1-165 (HA211248). |
| Positive control: | Recombinant Human TACI / TNFRSF13B protein (HA211248). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-66] to Human TACI / TNFRSF13B antibody (Capture) (HA725301) and Recombinant Human TACI / TNFRSF13B protein (HA211248) as the standard. The reference range value is 93.8-6,000 pg/mL. |
| Uniprot #: | SwissProt: O14836 Human |
| Alternative names: | Tumor necrosis factor receptor superfamily member 13B Transmembrane activator and CAML interactor CD267 TNFRSF13B TACI |
Images
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Fig1:
Sandwich ELISA analysis of human TNFRSF13B matched pair antibodies Capture: HA725301, Human TACI / TNFRSF13B Rabbit mAb [PSH18-66] Detector: HA725302, Human TACI / TNFRSF13B Rabbit mAb [PSH18-67] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725301) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TACI / TNFRSF13B protein (HA211248) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725302, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native TNFRSF13B in human serum samples. Capture: HA725301, Human TACI / TNFRSF13B Rabbit mAb [PSH18-66] Detector: HA725302, Human TACI / TNFRSF13B Rabbit mAb [PSH18-67] The concentrations of TNFRSF13B were measured in duplicates, interpolated from the TNFRSF13B standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF13B concentration was determined to be 193.177 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.