Human CD83 Recombinant Rabbit Monoclonal Antibody [PSH20-09]
cat.: HA725306
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH20-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD83 aa 20-143 (HA211203). |
| Positive control: | Recombinant Human CD83 protein (HA211203). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-08] to Human CD83 antibody (Capture) (HA725305) and Recombinant Human CD83 protein (HA211203) as the standard. The reference range value is 39.1-2,500 pg/mL. |
| Uniprot #: | SwissProt: Q01151 Human |
| Alternative names: | B cell activation 45kDa cell surface glycoprotein Ig superfamily B cell activation protein B-cell activation protein BL11 BL11 PEN CD83 CD83 antigen (activated B lymphocytes, immunoglobulin superfamily) CD83 antigen CD83 molecule CD83_HUMAN Cell surface glycoprotein Cell surface protein HB15 HB15 hCD83 |
Images
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Fig1:
Sandwich ELISA analysis of Human CD83 matched pair antibodies Capture: HA725305, Human CD83 Rabbit mAb [PSH20-08] Detector: HA725306, Human CD83 Rabbit mAb [PSH20-09] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725305) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD83 protein (HA211203) starting from 7,000 pg/ml to 0 pg/ml and detect antibody (HA725306, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native CD83 in Raji samples. Capture: HA725305, Human CD83 Rabbit mAb [PSH20-08] Detector: HA725306, Human CD83 Rabbit mAb [PSH20-09] The concentrations of CD83 were measured in duplicates, interpolated from the CD83 standard curve and corrected for sample dilution. Undiluted samples are Raji supernatant 100% and THP-1 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD83 concentration was determined to be 148 pg/ml in Raji. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.