AGRP Recombinant Rabbit Monoclonal Antibody [PSH21-43]
cat.: HA725308
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AGRP aa 21-132. |
| Positive control: | Recombinant Human AGRP protein. |
| Subcellular location: | Secreted, Golgi apparatus lumen. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-42] to AGRP antibody (Capture) (HA725307) and Recombinant Human AGRP protein as the standard. The reference range value is 156.3-10,000 pg/mL. |
| Uniprot #: | SwissProt: O00253 Human | P56473 Mouse |
| Alternative names: | Agouti-related protein Agrp Agrt Art |
Images
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Fig1:
Sandwich ELISA analysis of Human AGRP matched pair antibodies Capture: HA725307, AGRP Rabbit mAb [PSH21-42] Detector: HA725308, AGRP Rabbit mAb [PSH21-43] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725307) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human AGRP protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725308, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native AGRP in mouse thalamus(27d). Capture: HA725307, AGRP Rabbit mAb [PSH21-42] Detector: HA725308, AGRP Rabbit mAb [PSH21-43] The concentrations of mouse AGRP measured in duplicate and interpolated from the human AGRP standard curve and corrected for sample dilution. Undiluted samples are mouse thalamus(27d) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean mouse AGRP concentration was determined to be 5,508.58 pg/ml in mouse thalamus(27d). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.