Biotin Conjugated Human GRN Recombinant Rabbit Monoclonal Antibody [PSH22-43]
cat.: HA725310B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH22-43 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GRN aa 18-593 (HA211273). |
| Positive control: | Recombinant Human GRN protein (HA211273). |
| Subcellular location: | Secreted, Lysosome. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-42] to Human GRN antibody (Capture) (HA725309) and Recombinant Human GRN protein (HA211273) as the standard. The reference range value is 3.125-100 ng/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P28799 Human |
| Alternative names: | Acrogranin CLN11 GEP GP88 Granulin A Granulin B Granulin C Granulin D Granulin E Granulin epithelin Granulin F Granulin G Granulin-7 Granulins GRN GRN_HUMAN PC cell derived growth factor PCDGF PEPI PGRN Proepithelin Progranulin |
Images
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Fig1:
Sandwich ELISA analysis of GRN matched pair antibodies Capture: HA725309, Human GRN Rabbit mAb [PSH22-42] Detector: HA725310, Human GRN Rabbit mAb [PSH22-43] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725309) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GRN protein (HA211273) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725310, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Human GRN in human plasma samples. Capture: HA725309, Human GRN Rabbit mAb [PSH22-42] Detector: HA725310, Human GRN Rabbit mAb [PSH22-43] The concentrations of Human Factor B were measured in duplicates, interpolated from the Human Factor B standard curve and corrected for sample dilution. Undiluted samples are human plasma 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Human Factor B concentration was determined to be 24.827 ng/ml in human plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.