Human WNT7A Recombinant Rabbit Monoclonal Antibody [PSH19-61]
cat.: HA725313
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human WNT7A aa 32-349. |
| Positive control: | Recombinant Human WNT7A protein. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-62] to Human WNT7A antibody (Detector) (HA725314) and Recombinant Human WNT7A protein as the standard. The reference range value is 78.1-40,000 pg/mL. |
| Uniprot #: | SwissProt: O00755 Human |
| Alternative names: | Protein Wnt-7a Protein Wnt-7a precursor Proto oncogene Wnt7a protein proto-oncogene wnt7a protein wingless-type MMTV integration site family member 7A Wnt family member 7A WNT7A WNT7A_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human WNT7A matched pair antibodies Capture: HA725313, Human WNT7A Rabbit mAb [PSH19-61] Detector: HA725314, Human WNT7A Rabbit mAb [PSH19-62] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725313) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human WNT7A protein starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA725314, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native WNT7A in BxPC-3 extract samples based on a 1,000 µg/ml extract load. Capture: HA725313, Human WNT7A Rabbit mAb [PSH19-61] Detector: HA725314, Human WNT7A Rabbit mAb [PSH19-62] The concentrations of WNT7A were measured in duplicates, interpolated from the WNT7A standard curve and corrected for sample dilution. Undiluted samples are BxPC-3 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean WNT7A concentration was determined to be 2,669 pg/ml in BxPC-3. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.