Human IL-17F Recombinant Rabbit Monoclonal Antibody [PSH18-69]
cat.: HA725316
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-69 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-17F aa 31-163 (HA210716). |
| Positive control: | Recombinant Human IL-17F protein (HA210716). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-68] to Human IL-17F antibody (Capture) (HA725315) and Recombinant Human IL-17F protein (HA210716) as the standard. The reference range value is 39.1-20,000 pg/mL. |
| Uniprot #: | SwissProt: Q96PD4 Human |
| Alternative names: | CANDF6 Cytokine ML 1 Cytokine ML-1 IL 17F IL 24 IL-17F IL-24 Il17f IL17F_HUMAN Interleukin 17F Interleukin 24 Interleukin-17F Interleukin-24 ML 1 ML1 Mutant IL 17F OTTHUMP00000016602 |
Images
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Fig1:
Sandwich ELISA analysis of human IL17F matched pair antibodies Capture: HA725315, Human IL-17F Rabbit mAb [PSH18-68] Detector: HA725316, Human IL-17F Rabbit mAb [PSH18-69] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725315) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-17F protein (HA210716) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725316, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 12 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native IL17F in PHA Stim. PBMC Supernatant. Capture: HA725315, Human IL-17F Rabbit mAb [PSH18-68] Detector: HA725316, Human IL-17F Rabbit mAb [PSH18-69] The concentrations of IL17F were measured in duplicates, interpolated from the IL17F standard curve and corrected for sample dilution. Undiluted samples are 50% PHA-M(10ug/mL 5days) stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL17F concentration was determined to be 2817 pg/ml in PHA stimulated PBMC cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.