Human Lumican Recombinant Rabbit Monoclonal Antibody [PSH19-05]
cat.: HA725318
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human Lumican aa 19-338 (HA211171). |
| Positive control: | Recombinant Human Lumican protein (HA211171). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-04] to Human Lumican antibody (Capture) (HA725317) and Recombinant Human Lumican protein (HA211171) as the standard. The reference range value is 156.3-80,000 pg/mL. |
| Uniprot #: | SwissProt: P51884 Human |
| Alternative names: | Keratan sulfate proteoglycan lumican KSPG lumican LDC LUM LUM_HUMAN Lumican Lumican precursor SLRR2D |
Images
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Fig1:
Sandwich ELISA analysis of Human Lumican matched pair antibodies Capture: HA725317, Human Lumican Rabbit mAb [PSH19-04] Detector: HA725318, Human Lumican Rabbit mAb [PSH19-05] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725317) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Lumican protein (HA211171) starting from 80,000pg/ml to 0 pg/ml and detect antibody (HA725318, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Lumican in human serum samples. Capture: HA725317, Human Lumican Rabbit mAb [PSH19-04] Detector: HA725318, Human Lumican Rabbit mAb [PSH19-05] The concentrations of Lumican were measured in duplicates, interpolated from the Lumican standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lumican concentration was determined to be 172,905 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.