Human NCR1/NKp46 Recombinant Rabbit Monoclonal Antibody [PSH17-31]
cat.: HA725321
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NCR1/NKp46 aa 21-258 (HA211272). |
| Positive control: | Recombinant Human NCR1/NKp46 protein (HA211272). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-32] to Human NCR1/NKp46 antibody (Detector) (HA725322) and Recombinant Human NCR1/NKp46 protein (HA211272) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: O76036 Human |
| Alternative names: | Activating NK receptor NK p46 CD335 FLJ99094 hNKp46 Ly94 Lymphocyte antigen 94 Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) Lymphocyte antigen 94 homolog Natural cytotoxicity triggering receptor 1 Natural killer cell p46-related protein NCR1 NCT1 NCTR1_HUMAN NK cell activating receptor NK cell-activating receptor NK-p46 NKp46 |
Images
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Fig1:
Sandwich ELISA analysis of Human NCR1/NKp46 matched pair antibodies Capture: HA725321, Human NCR1/NKp46 Rabbit mAb [PSH17-31] Detector: HA725322, Human NCR1/NKp46 Rabbit mAb [PSH17-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725321) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NCR1/NKp46 protein (HA211272)starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725322, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native NCR1 in NK-92 cell culture supernatant. Capture: HA725321, Human NCR1/NKp46 Rabbit mAb [PSH17-31] Detector: HA725322, Human NCR1/NKp46 Rabbit mAb [PSH17-32] Interpolated concentration of native NCR1 was measured in duplicate at different sample concentrations and interpolated from the NCR1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean spp1 concentration was determined to be 533 pg/ml in NK-92 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.