Human MMP-12 Recombinant Rabbit Monoclonal Antibody [PSH22-92]
cat.: HA725323
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MMP-12 aa 280-470 (HA210856). |
| Positive control: | Recombinant Human MMP-12 protein (HA210856). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-93] to Human MMP-12 (Detecor) (HA725324) and Recombinant Human MMP-12 protein (HA210856) as the standard. The reference range value is 3.9-1,000 pg/mL. |
| Uniprot #: | SwissProt: P39900 Human |
| Alternative names: | EC 3.4.24.65 HME Macrophage elastase Macrophage metalloelastase Macrophage metaloelastase Matrix metallopeptidase 12 (macrophage elastase) Matrix metalloprotease 12 Matrix metalloproteinase-12 ME MGC138506 MME MMP 12 MMP-12 Mmp12 MMP12_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human MMP-12 matched pair antibodies Capture: HA725323, Human MMP-12 Rabbit mAb [PSH22-92] Detector: HA725324, Human MMP-12 Rabbit mAb [PSH22-93] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725323) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human MMP-12 protein (HA210856) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725324, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native MMP-12 in THP-1 cell extract: Capture: HA725323, Human MMP-12 Rabbit mAb [PSH22-92] Detector: HA725324, Human MMP-12 Rabbit mAb [PSH22-93] The concentrations of MMP-12 were measured in duplicates, interpolated from the MMP-12 standard curve and corrected for sample dilution. Undiluted samples are THP-1 cell extract 1 mg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-12 concentration was determined to be 41.7 pg/ml in THP-1 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.