Biotin Conjugated Human Flt3 Ligand / FLT3LG Recombinant Rabbit Monoclonal Antibody [PSH20-11]
cat.: HA725326B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Flt3 Ligand / FLT3LG aa 27-184 (HA211222). |
| Positive control: | Recombinant Human Flt3 Ligand / FLT3LG protein (HA211222). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-10] to Human Flt3 Ligand / FLT3LG antibody (Capture) (HA725325) and Recombinant Human Flt3 Ligand / FLT3LG protein (HA211222) as the standard. The reference range value is 7.8-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P49771 Human |
| Alternative names: | FL Flt 3 ligand Flt 3L Flt3 L FLT3 LG Flt3 ligand Flt3L FLT3L_HUMAN Flt3lg Fms related tyrosine kinase 3 ligand Fms-related tyrosine kinase 3 ligand SL cytokine |
Images
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Fig1:
Sandwich ELISA analysis of human FLT3LG matched pair antibodies Capture: HA725325, Human Flt3 Ligand / FLT3LG Rabbit mAb [PSH20-10] Detector: HA725326, Human Flt3 Ligand / FLT3LG Rabbit mAb [PSH20-11] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725325) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Flt3 Ligand / FLT3LG protein (HA211222) starting from 7,000 pg/ml to 0 pg/ml and detect antibody (HA725326, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native FLT3LG in human serum samples. Capture: HA725325, Human Flt3 Ligand / FLT3LG Rabbit mAb [PSH20-10] Detector: HA725326, Human Flt3 Ligand / FLT3LG Rabbit mAb [PSH20-11] The concentrations of FLT3LG were measured in duplicates, interpolated from the FLT3LG standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FLT3LG concentration was determined to be 130 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.