Human IKK gamma Recombinant Rabbit Monoclonal Antibody [PSH21-06]
cat.: HA725328
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human IKK gamma aa 1-272 (HA211330). |
| Positive control: | Recombinant Human IKK gamma protein (HA211330). |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-05] to Human IKK gamma antibody (Capture) (HA725327) and Recombinant Human IKK gamma protein (HA211330) as the standard. The reference range value is 195.3-25,000 pg/mL. |
| Uniprot #: | SwissProt: Q9Y6K9 Human |
| Alternative names: | IkB kinase associated protein 1 IkB kinase subunit gamma Inhibitor of nuclear factor kappa B kinase subunit gamma AMCBX1 FIP 3 FIP-3 FIP3 Fip3p I kappa B kinase gamma I-kappa-B kinase subunit gamma IkB kinase gamma subunit IkB kinase subunit gamma IkB kinase-associated protein 1 Ikbkg IKK-gamma IKKAP1 IKKG IMD33 Incontinentia pigmenti Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase gamma Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, gamma Inhibitor of nuclear factor kappa-B kinase subunit gamma IP IP1 IP2 IPD2 NEMO NEMO_HUMAN NF kappa B essential modifier NF kappa B essential modulator NF-kappa-B essential modifier NF-kappa-B essential modulator ZC2HC9 |
Images
|
Fig1:
Sandwich ELISA analysis of Human IKK gamma matched pair antibodies Capture: HA725327, Human IKK gamma Rabbit mAb [PSH21-05] Detector: HA725328, Human IKK gamma Rabbit mAb [PSH21-06] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725327) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IKK gamma protein (HA211330) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725328, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native IKK gamma in Hela and Jurkat cell culture lysates. Capture: HA725327, Human IKK gamma Rabbit mAb [PSH21-05] Detector: HA725328, Human IKK gamma Rabbit mAb [PSH21-06] The concentrations of IKK gamma were measured in duplicates, interpolated from the IKK gamma standard curve and corrected for sample dilution. Undiluted HeLa lysate is at 500 μg/ml and undiluted Jurkat lysate is at 250 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IKK gamma concentration was determined to be 16.438 ng/ml in Hela cell culture lysate and 29.60ng/ml in Jurkat cell culture lysate. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.