Human Galectin 9 Recombinant Rabbit Monoclonal Antibody [PSH21-15]
cat.: HA725333
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human Galectin 9 aa 2-323 (HA211310). |
| Positive control: | Recombinant Human Galectin 9 protein (HA211310). |
| Subcellular location: | Cytoplasm, Nucleus, Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-16] to Human Galectin 9 antibody (Detector) (HA725334) and Recombinant Human Galectin 9 protein (HA211310) as the standard. The reference range value is 78.125-10,000 pg/mL. |
| Uniprot #: | SwissProt: O00182 Human |
| Alternative names: | 36 kDa beta-galactoside-binding lectin Ecalectin Gal-9 galectin 9 Galectin-9 galectin9 HOM HD 21 HOMHD21 HUAT Lectin galactoside binding soluble 9 LEG9_HUMAN LGAL S9 LGALS 9 Lgals9 LGALS9A MGC117375 MGC125973 MGC125974 Tumor antigen HOM-HD-21 UAT Urate transporter/channel Urate transporter/channel protein |
Images
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Fig1:
Sandwich ELISA analysis of Human Galectin 9 matched pair antibodies Capture: HA725333, Human Galectin 9 Rabbit mAb [PSH21-15] Detector: HA725334, Human Galectin 9 Rabbit mAb [PSH21-16] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725333) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Galectin 9 protein (HA211310) starting from 7,000 pg/ml to 0 pg/ml and detect antibody (HA725334, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Galectin 9 in HT-29 samples. Capture: HA725333, Human Galectin 9 Rabbit mAb [PSH21-15] Detector: HA725334, Human Galectin 9 Rabbit mAb [PSH21-16] The concentrations of Galectin 9 were measured in duplicates, interpolated from the Galectin 9 standard curve and corrected for sample dilution. Undiluted samples are HT-29 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin 9 concentration was determined to be 971 pg/ml in HT-29. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.