Human Albumin Recombinant Rabbit Monoclonal Antibody [PSH27-49]
cat.: HA725335
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH27-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Albumin. |
| Positive control: | Recombinant Human Albumin protein. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH27-50] to Human Albumin (Detecor) (HA725336) and Recombinant Human Albumin protein as the standard. The reference range value is 97.7-25,000 pg/mL. |
| Uniprot #: | SwissProt: P02768 Human |
| Alternative names: | alb ALBU_HUMAN Albumin (32 AA) Albumin (AA 34) cell growth inhibiting protein 42 DKFZp779N1935 GIG20 GIG42 growth-inhibiting protein 20 OTTHUMP00000220436 OTTHUMP00000220438 OTTHUMP00000220439 PRO0883 PRO0903 PRO1341 PRO1708 PRO2044 PRO2619 PRO2675 Serum albumin UNQ696/PRO1341 |
Images
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Fig1:
Sandwich ELISA analysis of Human Albumin protein matched pair antibodies Capture: HA725335, Human Albumin Rabbit mAb [PSH27-49] Detector: HA725336, Human Albumin Rabbit mAb [PSH27-50] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725335) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Albumin protein starting from 25,000 pg/mL to 0 pg/mL and detect antibody (HA725336, Biotin, 0.05 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Albumin in HepG2 and HeLa cell supernatant. Capture: HA725335, Human Albumin Rabbit mAb [PSH27-49] Detector: HA725336, Human Albumin Rabbit mAb [PSH27-50] The concentrations of native Albumin measured in duplicate and interpolated from the Albumin standard curve and corrected for sample dilution. Undiluted samples are HepG2 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 1,577.02 ng/mL in HepG2 cell culture supernatant. There was no detectable signal in HeLa cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.