Biotin Conjugated Human CCL15/MIP-5 Recombinant Rabbit Monoclonal Antibody [PSH23-12]
cat.: HA725342B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH23-12 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL15/MIP-5 aa 22-113 (HA211207). |
| Positive control: | Recombinant Human CCL15/MIP-5 protein (HA211207). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-11] to Human CCL15/MIP-5 (Capture) (HA725341) and Recombinant Human CCL15/MIP-5 protein (HA211207) as the standard. The reference range value is 62.5-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q16663 Human |
| Alternative names: | C-C motif chemokine 15 C-C motif chemokine ligand 15 CC chemokine 3 CCL15 CCL15(29-92) CCL15_HUMAN Chemokine (C C motif) ligand 15 Chemokine CC-2 Chemokine CC2 HCC-2 HCC2 HMRP 2B HMRP2B Leukotactin 1 Leukotactin-1 LKN-1 LKN1 Macrophage inflammatory protein 5 MIP 1D MIP-1 delta MIP-1D MIP-5 MIP1 delta MIP1d MIP5 MRP 2B Mrp-2b Mrp2b NCC 3 NCC-3 NCC3 New CC chemokine 3 OTTHUMP00000163955 SCYA15 SCYL3 Small inducible cytokine A15 Small inducible cytokine subfamily A (Cys Cys) member 15 Small-inducible cytokine A15 SY15 |
Images
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Fig1:
Sandwich ELISA analysis of Human CCL15/MIP-5 matched pair antibodies Capture: HA725341, Human CCL15/MIP-5 Rabbit mAb [PSH23-11] Detector: HA725342, Human CCL15/MIP-5 Rabbit mAb [PSH23-12] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725341) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL15/MIP-5 protein (HA211207) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725342, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL15/MIP-5 in human serum samples. Capture: HA725341, Human CCL15/MIP-5 Rabbit mAb [PSH23-11] Detector: HA725342, Human CCL15/MIP-5 Rabbit mAb [PSH23-12] The concentrations of CCL15/MIP-5 were measured in duplicates, interpolated from the CCL15/MIP-5 standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL15/MIP-5 concentration was determined to be 6,290 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.