Biotin Conjugated Human CD127 Recombinant Rabbit Monoclonal Antibody [PSH18-28]
cat.: HA725346B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH18-28 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD127 aa 21-239 (HA211291). |
| Positive control: | Recombinant Human CD127 protein (HA211291). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-27] to Human CD127 antibody (Capture) (HA725345) and Recombinant Human CD127 protein (HA211291) as the standard. The reference range value is 11.7-6,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P16871 Human |
| Alternative names: | CD 127 CD127 CD127 antigen CDw127 IL 7R alpha IL 7R IL-7 receptor subunit alpha IL-7R subunit alpha IL-7R-alpha IL-7RA IL7R IL7RA IL7RA_HUMAN IL7Ralpha ILRA Interleukin 7 receptor alpha chain Interleukin 7 receptor Interleukin 7 receptor isoform H5 6 Interleukin-7 receptor subunit alpha |
Images
|
Fig1:
Sandwich ELISA analysis of Human CD127 matched pair antibodies Capture: HA725345, Human CD127 Rabbit mAb [PSH18-27] Detector: HA725346, Human CD127 Rabbit mAb [PSH18-28] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725345) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD127 protein (HA211291) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA725346, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native CD127 in human serum samples. Capture: HA725345, Human CD127 Rabbit mAb [PSH18-27] Detector: HA725346, Human CD127 Rabbit mAb [PSH18-28] The concentrations of CD127 were measured in duplicates, interpolated from the CD127 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD127 concentration was determined to be 6413 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.