Human SIRP alpha Recombinant Rabbit Monoclonal Antibody [PSH17-64]
cat.: HA725348
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH17-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SIRP alpha aa 31-369 (HA211204). |
| Positive control: | Recombinant Human SIRP alpha protein (HA211204). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-63] to Human SIRP alpha antibody (Capture) (HA725347) and Recombinant Human SIRP alpha protein (HA211204) as the standard. The reference range value is 156-40,000 pg/mL. |
| Uniprot #: | SwissProt: P78324 Human |
| Alternative names: | Signal regulatory protein alpha type 1 Bit Brain Ig like molecule with tyrosine based activation motifs Brain Ig-like molecule with tyrosine-based activation motifs Brain immunoglobulin like molecule with tyrosine based activation motifs CD172 antigen like family member A CD172 antigen-like family member A CD172a CD172a antigen Inhibitory receptor SHPS-1 Macrophage fusion receptor MFR MYD 1 Myd 1 antigen MyD-1 antigen p84 Protein tyrosine phosphatase non receptor type substrate 1 PTPNS1 SHP substrate 1 SHPS-1 SHPS1 SHPS1_HUMAN Signal regulatory protein alpha 2 Signal regulatory protein alpha 3 Signal regulatory protein alpha Signal regulatory protein alpha type 2 Signal-regulatory protein alpha-1 Signal-regulatory protein alpha-2 Signal-regulatory protein alpha-3 SIRP Sirp-alpha-1 Sirp-alpha-2 Sirp-alpha-3 SIRPA SIRPalpha SIRPalpha1 SIRPalpha2 SIRPalpha3 Tyrosine phosphatase SHP substrate 1 Tyrosine protein p...... |
Images
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Fig1:
Sandwich ELISA analysis of Human SIRP alpha matched pair antibodies Capture: HA725347, Human SIRP alpha Rabbit mAb [PSH17-63] Detector: HA725348, Human SIRP alpha Rabbit mAb [PSH17-64] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725347) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human SIRP alpha (HA211204) protein starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA725348, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native SIRPA in U-937 cell extract and SH-SY5Y cell extract: Capture: HA725347, Human SIRP alpha Rabbit mAb [PSH17-63] Detector: HA725348, Human SIRP alpha Rabbit mAb [PSH17-64] The concentrations of SIRPA were measured in duplicates, interpolated from the SIRPA standard curve and corrected for sample dilution. Undiluted samples are U-937 cell extract 400 µg/mL and SH-SY5Y cell extract 400 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SIRPA concentration was determined to be 73,992 pg/ml in U-937 cell culture supernatant and 4,844 pg/ml in SH-SY5Y cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.