Human CCL4 / MIP-1 beta Recombinant Rabbit Monoclonal Antibody [PSH19-49]
cat.: HA725356
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL4 / MIP-1 beta aa 24-92 (HA211213). |
| Positive control: | Recombinant Human CCL4 / MIP-1 beta protein (HA211213). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-48] to Human CCL4 / MIP-1 beta antibody (Capture) (HA725355) and Recombinant Human CCL4 / MIP-1 beta protein (HA211213) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P13236 Human |
| Alternative names: | MIP 1 beta Secreted protein G 26 ACT 2 ACT-2 ACT2 AT744.1 AT744.2 C C motif chemokine 4 C C motif chemokine 4 like C C motif chemokine ligand 4 like 1 C C motif chemokine ligand 4 like 2 CC chemokine ligand 4 CC chemokine ligand 4L1 CC chemokine ligand 4L1d2 CC chemokine ligand 4L2 CCL4 CCL4_HUMAN ccl4l 1 CCL4L CCL4L1 Chemokine (C C motif) ligand 4 Chemokine (C C motif) ligand 4 like 1 Chemokine (C C motif) ligand 4 like 1, telomeric Chemokine (C C motif) ligand 4 like 2 Chemokine CC Motif Ligand 4 G 26 G 26 T lymphocyte secreted protein G-26 T-lymphocyte-secreted protein HC21 Immune activation 2 LAG 1 LAG-1 LAG1 Lymphocyte activation gene 1 Lymphocyte activation gene 1 protein Macrophage inflammatory protein 1 beta Macrophage inflammatory protein 1-beta Macrophage inflammatory protein 1b2 MGC104418 MGC126025 MGC126026 MIP-1-beta MIP-1-beta(1-69) MIP-1-beta(3-69) MIP1 beta MIP1B MIP1B1 Monocyt...... |
Images
|
Fig1:
Sandwich ELISA analysis of Human CCL4 / MIP-1 beta matched pair antibodies Capture: HA725355, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-48] Detector: HA725356, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-49] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725355) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL4 / MIP-1 beta protein (HA211213) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725356, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native CCL4 in TPA and LPS stimulated or unstimulated THP-1 cell culture supernatant. Capture: HA725355, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-48] Detector: HA725356, Human CCL4 / MIP-1 beta Rabbit mAb [PSH19-49] THP-1 cells were stimulated overnight with 100 nM TPA, and then stimulated with 100 ng/ml LPS for 7 hours. The concentrations of human CCL4 measured in duplicate and interpolated from the human CCL4 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 5% and stimulated 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human CCL4 concentration was determined to be 13,170.81 pg/ml in TPA and LPS stimulated THP-1 cell culture supernatant and undetectable in the unstimulated THP-1 control. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.