Human MCP-3/CCL7 Recombinant Rabbit Monoclonal Antibody [PSH22-95]
cat.: HA725360
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-95 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MCP-3/CCL7 aa 24-99 (HA211264). |
| Positive control: | Recombinant Human MCP-3/CCL7 protein (HA211264). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-94] to Human MCP-3/CCL7 (Capture) (HA725359) and Recombinant Human MCP-3/CCL7 protein (HA211264) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: P80098 Human |
| Alternative names: | C-C motif chemokine 7 Ccl7 CCL7_HUMAN Chemokine CC motif ligand 7 FIC MARC MCP-3 Monocyte chemoattractant protein 3 Monocyte chemotactic protein 3 NC28 RP23-350G1.4 SCYA6 SCYA7 Small-inducible cytokine A7 |
Images
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Fig1:
Sandwich ELISA analysis of Human MCP-3/CCL7 matched pair antibodies Capture: HA725359, Human MCP-3/CCL7 Rabbit mAb [PSH22-94] Detector: HA725360, Human MCP-3/CCL7 Rabbit mAb [PSH22-95] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725359) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MCP-3/CCL7 protein (HA211264) starting from 500 pg/ml to 0 pg/ml and detect antibody (HA725360, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.